Identification of Potential Target Transcription Factor Genes Regulated by Krüppel Homolog 1 in <i>Chilo suppressalis</i> (Lepidoptera: Crambidae)

Abstract Juvenile hormone (JH) is a major endocrine that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor recruits heterodimer partner form the JH–receptor complex then activates JH-inducible gene known as Krüppel homolog 1 (Kr-h1). There evidence this downstream factor mediating both physiological biochemical processes; however, functional mechanism of Kr-h1 largely unknown. Using economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) model, we used combination RNA interference (RNAi), high-throughput sequencing, real-time quantitative polymerase chain reaction (RT-qPCR) identify candidate transcription (TF) genes are regulated by Kr-h1. RNAi knockdown Krh1 identified Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, MBD families RT-qPCR confirmed levels these putative TFs indicated can induce or suppress expression proteins C. suppressalis. These results provide information required for in-depth research on other